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Forage nitrate analysis: laboratory performance study.

T L McMahon, H H Casper

Journal - Association of Official Analytical Chemists

|September 1, 1984

Summary

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Laboratory analysis of forage for potassium nitrate (KNO3) content showed significant variability, yielding unacceptable results for livestock feeding recommendations. Some reported values could lead to potentially toxic forage consumption.

Area of Science:

Agricultural Science
Analytical Chemistry
Veterinary Toxicology

Background:

Accurate quantification of potassium nitrate (KNO3) in forage is crucial for livestock health and nutrition.
Variability in analytical results can lead to misinformed feeding practices and potential toxicity.

Purpose of the Study:

To assess the reliability and consistency of laboratory analyses for KNO3 content in forage samples.
To evaluate the suitability of reported analytical data for making safe livestock feeding recommendations.

Main Methods:

Four forage samples were subjected to potassium nitrate (KNO3) analysis by sixteen independent laboratories.
Statistical analysis of reported KNO3 values, including calculation of coefficients of variation.
Assessment of KNO3 recovery from spiked samples to determine analytical accuracy.

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Main Results:

High coefficients of variation (36.0%, 51.0%, 12.8%, 55.0%) were observed across laboratories for the four forage samples.
Average recovery of spiked KNO3 was 105%, indicating potential overestimation or procedural issues.
Analytical results were deemed statistically unacceptable and unsuitable for feeding guidelines.

Conclusions:

The inter-laboratory variability in KNO3 analysis renders the data unreliable for practical agricultural applications.
Certain reported analytical values posed a risk of livestock consuming potentially toxic levels of KNO3.
Improved standardization and quality control in forage analysis are necessary to ensure animal safety and effective nutrition management.