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Difference spectrophotometric estimation of santonin.

A A Habib, A A Omar, T M Sarg

    Journal - Association of Official Analytical Chemists
    |September 1, 1984
    PubMed
    Summary
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    A new method uses the alkaline-acidic difference spectrum of Santonin for its accurate estimation in pharmaceuticals and crude drugs. This spectrophotometric technique provides a reliable way to quantify Santonin content.

    Area of Science:

    • Analytical Chemistry
    • Pharmacognosy

    Background:

    • Santonin is a key compound with pharmaceutical applications.
    • Accurate quantification of Santonin is crucial for quality control in drug manufacturing and natural product analysis.

    Purpose of the Study:

    • To develop and validate a spectrophotometric method for estimating Santonin.
    • To enable accurate quantification of Santonin in both pharmaceutical formulations and crude drug extracts.

    Main Methods:

    • Extraction and purification of Santonin using a specific partition procedure.
    • Measurement of alkaline vs. acidic difference spectra, focusing on absorbance at 285 nm (maximum) and 242 nm (minimum).
    • Calculation of Santonin percentage using reference standards or determined absorptivity.

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    Main Results:

    • The alkaline vs. acidic difference spectrum of Santonin was characterized.
    • The method demonstrated utility for Santonin estimation in pharmaceuticals and crude drugs.
    • Measurement at 285 nm is recommended for crude drug analysis due to potential interference at 242 nm.

    Conclusions:

    • The developed spectrophotometric method is effective for Santonin quantification.
    • The method's reliability is suitable for pharmaceutical quality control and natural product analysis.
    • Choosing the appropriate wavelength (285 nm) enhances accuracy when analyzing complex matrices like crude drugs.