Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Aromatic acids are chemoattractants for Pseudomonas putida.

C S Harwood, M Rivelli, L N Ornston

    Journal of Bacteriology
    |November 1, 1984
    PubMed
    Summary

    Pseudomonas putida PRS2000 uses aromatic acids as chemoattractants, not chemorepellents. This chemotaxis is inducible and involves distinct chemoreceptors, independent of aromatic acid degradation pathways.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Dependence of linkage of alleles on their physical distance in natural transformation of Acinetobacter sp. strain ADP1.

    Archives of microbiology·2001
    Same author

    Functions of the mismatch repair gene mutS from Acinetobacter sp. strain ADP1.

    Journal of bacteriology·2001
    Same author

    Cloning and genetic characterization of dca genes required for beta-oxidation of straight-chain dicarboxylic acids in Acinetobacter sp. strain ADP1.

    Applied and environmental microbiology·2001
    Same author

    Spontaneous mutations affecting transcriptional regulation by protocatechuate in Acinetobacter.

    FEMS microbiology letters·2001
    Same author

    Tryptase-induced airway microvascular leakage in guinea pigs: involvement of tachykinins and leukotrienes.

    European journal of pharmacology·2001
    Same author

    Reductive, coenzyme A-mediated pathway for 3-chlorobenzoate degradation in the phototrophic bacterium Rhodopseudomonas palustris.

    Applied and environmental microbiology·2001

    Area of Science:

    • Microbiology
    • Chemotaxis
    • Bacterial Physiology

    Background:

    • Aromatic acids are typically chemorepellents for bacteria like Escherichia coli.
    • Understanding bacterial chemotaxis is crucial for microbial ecology and biotechnology.
    • Pseudomonas putida is known for its metabolic versatility, including aromatic compound degradation.

    Purpose of the Study:

    • To investigate the chemotactic responses of Pseudomonas putida PRS2000 to aromatic acids.
    • To identify specific aromatic acids that act as chemoattractants for P. putida.
    • To elucidate the mechanisms and regulation of chemotaxis towards aromatic compounds.

    Main Methods:

    • Quantitative capillary assays were employed to measure chemotactic responses.
    • Bacterial chemotaxis was assessed using various aromatic acids as attractants.
    • Inducibility of chemotactic responses was investigated by pre-culturing cells on specific compounds.

    Main Results:

    • Aromatic acids, including benzoate and toluates, were identified as chemoattractants for P. putida PRS2000.
    • Chemotactic responses were inducible, with specific attractants being induced by metabolic intermediates or related compounds.
    • P. putida PRS2000 possesses at least two distinct chemoreceptors for aromatic acids, and chemotaxis is largely independent of degradation pathways.

    Conclusions:

    • Pseudomonas putida PRS2000 exhibits specific and inducible chemotaxis towards a range of aromatic acids.
    • The bacterium utilizes distinct chemoreceptors, suggesting a sophisticated sensory system for aromatic compounds.
    • Chemotaxis is not directly linked to the energetic benefits of aromatic acid oxidation, highlighting a role in nutrient seeking or environmental sensing.

    Related Experiment Videos