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Related Experiment Videos

Cell cycle regulation by environmental pH.

I W Taylor, P J Hodson

    Journal of Cellular Physiology
    |December 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Tumor cell proliferation is regulated by extracellular pH. Acidic conditions can halt cell cycle progression, suggesting pH-dependent therapeutic targets for cancer treatment.

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    Area of Science:

    • Cell Biology
    • Cancer Research
    • Biochemistry

    Background:

    • Quiescent tumor cells can re-enter the cell cycle.
    • Extracellular pH is a critical factor in cellular processes.

    Purpose of the Study:

    • To investigate the effect of extracellular pH on tumor cell cycle recruitment and growth.
    • To identify potential pH-dependent regulatory points in the tumor cell cycle.

    Main Methods:

    • Isolation of quiescent human tumor cells (PMC-22) using centrifugal elutriation.
    • Exposure of exponentially growing and quiescent tumor cells to varying extracellular pH levels.
    • Analysis of cell cycle progression and DNA content.

    Main Results:

    • Quiescent cells entered S phase after a lag period with increased RNA content.

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  • Acidic extracellular pH (below 6.8) reduced tumor cell growth rate.
  • A G1 DNA content accumulation occurred between pH 6.7 and 6.4.
  • Non-cell-cycle-phase-specific growth arrest was observed below pH 6.3.
  • Evidence suggests a pH-dependent restriction point in the G1 phase of tumor cells.

    • Conclusions:

    • Extracellular pH significantly regulates tumor cell cycle progression.
    • Acidic tumor microenvironments may contribute to growth arrest.
    • Targeting pH-dependent mechanisms could offer novel cancer therapeutic strategies.