The molecular mass of adenovirus type 5 as determined by means of scanning transmission electron microscopy (STEM)

The mass of adenovirus type 5 was determined by means of computer-assisted scanning transmission electron microscopy (STEM). Arithmetic mean of 157 +/- 10(SD) X 10(6) daltons and mode between 160 and 170 X 10(6) daltons compare favourably with previously reported data. The advantages of the STEM-procedure over the physical and chemical techniques are: low amounts of purified virus particles are needed; visual control of the physical state of virus particles; no need to know the chemical composition or protein concentration of the virus sample.