Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Thyroid hormone binding in rat liver cytosol.

G Morgan

    The Journal of Endocrinology
    |December 1, 1984
    PubMed
    Summary

    Thyroid hormones thyroxine (T4) and tri-iodothyronine (T3) bind to albumin in rat liver cells. Albumin is identified as a key T4-binding protein within hepatocytes, contributing to the liver

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Assessment of proprietary and improvised pelvic binders: time to application and displacement during casualty evacuation.

    BMJ military health·2025
    Same author

    Air pollution mortality benefits of sustained COVID-19 mobility restrictions in Australian cities.

    Public health·2023
    Same author

    Mechanical assessment of proprietary and improvised pelvic binders for use in the prehospital environment.

    BMJ military health·2023
    Same author

    Challenges and knowledge gaps with immune checkpoint inhibitors monotherapy in the management of patients with non-small-cell lung cancer: a survey of oncologist perceptions.

    ESMO open·2023
    Same author

    Experiences of Female Partners of People Transitioning Gender: A Feminist Interpretive Metasynthesis.

    Journal of sex & marital therapy·2022
    Same author

    The impact of COVID-19 on oncology professionals-one year on: lessons learned from the ESMO Resilience Task Force survey series.

    ESMO open·2022

    Area of Science:

    • Endocrinology
    • Molecular Biology
    • Hepatology

    Background:

    • Thyroid hormones, thyroxine (T4) and tri-iodothyronine (T3), are crucial for metabolic regulation.
    • Understanding the intracellular binding proteins for T3 and T4 in the liver is essential for comprehending thyroid hormone action.
    • Previous studies have identified various binding proteins in liver fractions, but their specific roles in vivo require further elucidation.

    Purpose of the Study:

    • To investigate the binding characteristics of T3 and T4 to soluble proteins in rat liver supernatant and cytosol.
    • To identify the specific binding proteins responsible for T3 and T4 uptake within liver cells.
    • To assess the physiological relevance of identified binding proteins for hepatic thyroid hormone transport and storage.

    Main Methods:

    • Gel chromatography was employed to separate binding fractions in rat liver supernatant.
    • Isolated liver cells were used to prepare cytosol for examining hormone binding patterns.
    • Albumin was identified as a major binding protein in specific fractions using these methods.

    Main Results:

    • Both T3 and T4 preferentially bound to the A-fraction in liver supernatant, which contained albumin.
    • In isolated liver cell cytosol, T4 predominantly bound to the A-fraction (albumin), while T3 preferentially bound to the Y-region.
    • Albumin was confirmed as a significant in vivo T4-binding protein within hepatocytes.

    Conclusions:

    • Albumin is a key intracellular protein in hepatocytes capable of binding T4.
    • The presence of albumin within liver cells likely contributes to the liver's high capacity for T4 binding compared to other tissues.
    • Hormone binding patterns in isolated cell cytosol may offer a more accurate reflection of in vivo conditions than liver supernatant.

    Related Experiment Videos