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Volume regulation by Necturus gallbladder: basolateral KCl exit.

M Larson, K R Spring

    The Journal of Membrane Biology
    |January 1, 1984
    PubMed
    Summary
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    Necturus gallbladder epithelial cells rapidly adjust volume after osmotic swelling. This process involves potassium chloride (KCl) exiting the cell via the basolateral membrane, specifically a 3K:2Cl stoichiometry.

    Area of Science:

    • Cell Biology
    • Physiology
    • Epithelial Transport

    Background:

    • Epithelial cells swell when exposed to hypotonic solutions.
    • Cells possess mechanisms to regulate their volume following osmotic stress.

    Purpose of the Study:

    • To investigate the ionic mechanisms and stoichiometry of volume regulatory decrease in Necturus gallbladder epithelial cells.
    • To identify the specific ion transporters involved in volume regulation.

    Main Methods:

    • Induction of cell swelling by reducing mucosal bath osmolality.
    • Measurement of intracellular potassium (K) and chloride (Cl) activities.
    • Assessment of volume regulation rates under various ionic gradient conditions.
    • Inhibition studies using bumetanide.

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    Main Results:

    • Volume regulatory decrease requires chloride (Cl) and is influenced by basolateral K and Cl gradients.
    • The process is insensitive to amiloride, SITS, ouabain, and bicarbonate removal.
    • Bumetanide in the serosal bath blocked volume regulatory decrease.
    • A 3K:2Cl stoichiometry for KCl exit across the basolateral membrane was determined.

    Conclusions:

    • Volume regulatory decrease in Necturus gallbladder is mediated by a K-Cl cotransporter on the basolateral membrane.
    • This transport mechanism is activated transiently following hypotonic shock.