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Updated: Aug 18, 2026

Detecting, Visualizing and Quantitating the Generation of Reactive Oxygen Species in an Amoeba Model System
Published on: November 5, 2013
Abstract:
Autoxidation of Acholeplasma laidlawii membranes (with equimolar ratio of palmitic and linoleic acid) lacks an obvious induction period, and the overall rate of disappearance of substrate does not follow closely that of typical autocatalytic kinetics. Throughout the course of autoxidation, the major oxygenated products isolated were hydroperoxides (as hydroxy esters) and compounds that gave rise to trihydroxy esters. The yield of trihydroxy esters was appreciable even at the early stage of the oxidation and eventually grew to surpass that of hydroperoxides. The positions of the three hydroxyl groups in the trihydroxy esters were determined to be mostly of the 1,2,5-type rather than 1,2,3-type arrangement. To a lesser extent, some degraded products, including dimethyl nonanedioate, methyl myristate, methyl pentadecanoate, methyl hexadecadienoate and methyl heptadecadienoate also were obtained. Dimethyl nonanedioate was a previously known degradation product from 9-hydroperoxide. The shorter chain esters presumably arise from the cleavage of alpha-hydroperoxides of palmitate and linoleate moieties.
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