Related Experiment Video
Updated: Aug 2, 2026

15:57
Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
Published on: July 11, 2015
Summary
Grain-fed heifers experienced stiffness and sternal recumbency after winter confinement. Selenium-vitamin E treatment and calcium-magnesium infusions led to recovery, suggesting a nutritional deficiency.
Area of Science:
- Veterinary Medicine
- Animal Nutrition
- Biochemistry
Background:
- Winter confinement can lead to nutritional deficiencies in livestock.
- Exercise can precipitate clinical signs of underlying metabolic disorders in cattle.
- Grain-fed diets may alter nutrient absorption or requirements.
Purpose of the Study:
- To investigate the cause of stiffness and recumbency in grain-fed heifers.
- To evaluate the efficacy of specific treatments for affected cattle.
- To explore potential links between diet, confinement, and clinical presentation.
Main Methods:
- Observation of clinical signs in a group of heifers post-confinement.
- Administration of intramuscular (IM) injections of selenium-vitamin E.
- Intravenous (IV) infusion of a calcium-magnesium solution.
- Serum assays to assess selenium levels.
Main Results:
- Affected heifers exhibited stiffness and sternal recumbency upon exercise.
- All but two heifers recovered following IM selenium-vitamin E and IV calcium-magnesium treatment.
- Serum selenium levels were not detected as low, despite clinical response to treatment.
Conclusions:
- Clinical signs suggested a presumptive diagnosis of selenium deficiency, despite inconclusive serum assays.
- Selenium-vitamin E supplementation and calcium-magnesium infusions were effective in treating the affected heifers.
- Further investigation into nutritional status and metabolic responses in confined cattle is warranted.

