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Quantitative evaluation of a transport-enrichment medium for Campylobacter fetus
Abstract:
The enrichment feature of a selective serum-based transport medium for Campylobacter fetus was quantitatively examined. Preputial samples from artificial insemination bulls were spiked with known numbers of C fetus strains and inoculated into transport-enrichment medium (TEM). The survival and multiplication of these strains in TEM under different incubation periods and temperatures were assessed by plate counts. Mean enrichment values of 3.72 log and 4.42 log were observed after incubation at 37 degrees C for two and four days, respectively. There was no significant difference in the enrichment values between the C fetus subspecies venerealis strains and a C fetus subspecies fetus strain. Incubation of inoculated TEM vials at room temperature for up to two days neither improved the growth of C fetus nor affected its subsequent enrichment when the vials were reincubated at 37 degrees C. Comparison of the survival of C fetus with and without the use of TEM under simulated transport conditions demonstrated the superiority of TEM.
Insights
This study demonstrates that a selective serum-based transport-enrichment medium (TEM) significantly enhances Campylobacter fetus survival and multiplication in bull prepuce samples. TEM is superior for preserving C. fetus during simulated transport conditions.
Area of Science:
- Veterinary Microbiology
- Bacterial Pathogenesis
- Reproductive Health
Background:
- Campylobacter fetus is a significant pathogen affecting bovine reproduction.
- Effective sample transport is crucial for accurate diagnosis of C. fetus infections.
Purpose of the Study:
- To quantitatively assess the enrichment capabilities of a selective serum-based transport-enrichment medium (TEM) for Campylobacter fetus.
- To evaluate the survival and multiplication of C. fetus strains within TEM under various conditions.
Main Methods:
- Preputial samples from artificial insemination bulls were artificially contaminated with C. fetus strains.
- Samples were inoculated into TEM and incubated under different temperatures and durations.
- Bacterial enumeration was performed using plate counts to determine enrichment values.
Main Results:
- Significant C. fetus enrichment was observed, with mean values of 3.72 log after 2 days and 4.42 log after 4 days at 37°C.
- No significant difference in enrichment was found between C. fetus subspecies venerealis and C. fetus subspecies fetus strains.
- Incubation at room temperature did not enhance C. fetus growth in TEM, nor did it negatively impact subsequent enrichment at 37°C.
Conclusions:
- The selective serum-based TEM is highly effective for the enrichment and preservation of Campylobacter fetus.
- TEM demonstrates superior performance compared to standard methods for maintaining C. fetus viability under simulated transport conditions.
- The findings support the use of TEM for improved diagnostic accuracy of C. fetus in veterinary samples.