Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Differences in threshold for protamine toxicity in isolated atrial and ventricular tissue.

R A Caplan, J Y Su

    Anesthesia and Analgesia
    |December 1, 1984
    PubMed
    Summary

    Protamine sulfate significantly impacts rabbit heart tissue, causing dose-dependent decreases in tension. This study reveals a narrow safety margin for protamine, potentially explaining clinical hypotension.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Spatiotemporal characteristics of neural activity in tibial nerves with carbon nanotube yarn electrodes.

    Journal of neuroscience methods·2019
    Same author

    [Advance in the research of graphene and its derivatives in wound dressings].

    Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns·2019
    Same author

    [Study of iron overload assessment by T2* magnetic resonance imaging in patients with myelodysplastic syndromes].

    Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2019
    Same author

    Dietary supplementation with arginine and glutamic acid enhances key lipogenic gene expression in growing pigs.

    Journal of animal science·2018
    Same author

    [miR-202 contributes to sensitizing MM cells to drug significantly via activing JNK/SAPK signaling pathway].

    Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2016
    Same author

    [Study of abnormal iron metabolism parameters and iron overload in patients with myelodysplastic syndromes].

    Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2016

    Area of Science:

    • Cardiovascular Pharmacology
    • Cardiac Electrophysiology
    • Myocardial Contractility

    Background:

    • Protamine sulfate is used to neutralize heparin during cardiac surgery.
    • Understanding its direct effects on cardiac tissue is crucial for patient safety.

    Purpose of the Study:

    • To investigate the inotropic and chronotropic effects of protamine sulfate on isolated rabbit atrial and ventricular myocardium.
    • To determine the dose-response relationship and susceptibility of different cardiac tissues to protamine sulfate.

    Main Methods:

    • Isolated rabbit atrial and ventricular tissues were used to assess the effects of varying protamine sulfate concentrations.
    • Measurements included peak isometric developed tension and electrical activity.

    Related Experiment Videos

    Main Results:

    • Different cardiac tissues exhibited varying sensitivity to protamine sulfate.
    • Left atrium was most sensitive (0.022% depression), followed by right atrium (0.055%) and right ventricular papillary muscle (0.3%).
    • Steep dose-response curves were observed, with significant tension depression at low concentrations and intermittent atrial extrasystoles noted.

    Conclusions:

    • Protamine sulfate exerts significant, dose-dependent inotropic effects on rabbit myocardium.
    • The narrow margin of safety in protamine's dose-response curve may contribute to observed hypotensive episodes during clinical use.