Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rapid diagnostic DNA cytometry with an automatic microscope and a TV image-analysis system.

W Auffermann, R Repges, A Böcking

    Analytical and Quantitative Cytology
    |September 1, 1984
    PubMed
    Summary

    This study introduces rapid DNA cytometry for cancer diagnosis using an automated microscope system. The technology assists cytopathologists in diagnosing and grading tumors, improving diagnostic accuracy and efficiency.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Wavelet-based scaling indices for breast cancer diagnostics.

    Statistics in medicine·2017
    Same author

    [Objective DNA malignancy grading as adjunct to the histological Gleason score: Frankfurt consensus].

    Der Pathologe·2015
    Same author

    Fetal sex and preterm birth.

    Placenta·2012
    Same author

    Treatment of premenstrual tension syndrome with Vitex agnus castus controlled, double-blind study versus pyridoxine.

    Phytomedicine : international journal of phytotherapy and phytopharmacology·2012
    Same author

    [Prevention, diagnosis, therapy, and follow-up of lung cancer. Interdisciplinary guideline of the German Respiratory Society and the German Cancer Society--abridged version].

    Pneumologie (Stuttgart, Germany)·2011
    Same author

    Prevention, diagnosis, therapy, and follow-up of lung cancer: interdisciplinary guideline of the German Respiratory Society and the German Cancer Society.

    Pneumologie (Stuttgart, Germany)·2010

    Area of Science:

    • Cytopathology
    • Medical image analysis
    • Biomedical engineering

    Background:

    • Accurate cancer diagnosis and grading are crucial for effective treatment.
    • Traditional cytopathology methods can be time-consuming and subjective.
    • Automated systems offer potential for increased efficiency and objectivity in diagnostic procedures.

    Purpose of the Study:

    • To evaluate a TV-based image-analysis system (Leitz TAS plus) and automatic microscope for rapid DNA cytometry.
    • To develop and validate an automated program for nuclear DNA content measurement.
    • To assess the system's utility in routine cytology for diagnosis and tumor grading.

    Main Methods:

    • Utilized a Leitz TAS plus system and automatic microscope for image analysis.
    • Developed an algorithm for automatic detection and measurement of nuclear DNA content.

    Related Experiment Videos

  • Cells identified as malignant or suspicious by cytopathologists were automatically relocated and measured.

    • Main Results:

    • The system enabled rapid DNA cytometry for diagnostic purposes.
    • The automated procedure demonstrated high reproducibility in real-time measurements.
    • The algorithm successfully processed DNA data for malignancy diagnosis and grading.

    Conclusions:

    • Diagnostic DNA cytometry can be integrated into routine cytology laboratories.
    • The automated system assists cytopathologists, particularly in cases of diagnostic uncertainty and tumor grading.
    • This technology enhances diagnostic capabilities without replacing the role of the cytopathologist.