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Related Experiment Videos

Atriopeptin release from the isolated perfused rabbit heart.

M G Currie, D Sukin, D M Geller

    Biochemical and Biophysical Research Communications
    |November 14, 1984
    PubMed
    Summary

    Mammalian hearts release low molecular weight atrial peptides, not their high molecular weight precursors. This suggests atrial peptides are processed before release, impacting natriuretic and diuretic functions.

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    Area of Science:

    • Cardiovascular Physiology
    • Endocrinology
    • Biochemistry

    Background:

    • Mammalian atrial extracts contain bioactive peptides with natriuretic, diuretic, and smooth muscle relaxant effects.
    • These include low molecular weight atrial peptides (atriopeptins) derived from a high molecular weight precursor (atriopeptigen).

    Purpose of the Study:

    • To investigate the molecular forms of bioactive atrial peptides released from the heart.
    • To determine if the precursor peptide (atriopeptigen) or processed peptides (atriopeptins) are released.

    Main Methods:

    • Extraction and purification of bioactive peptides from rabbit atria.
    • Perfusion of isolated rabbit hearts and analysis of coronary venous effluent.
    • Gel filtration chromatography and reversed-phase High-Performance Liquid Chromatography (HPLC) for separation and identification.

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  • Assay strips (rabbit aorta, chicken rectum) to measure biological activity.

    • Main Results:

    • Rabbit atria contain both high and low molecular weight bioactive atrial peptides.
    • Coronary venous effluent from isolated perfused rabbit hearts exclusively contained low molecular weight atrial peptides.
    • The released peptide exhibited trypsin lability and caused dose-dependent relaxation of assay strips.
    • Chromatographic separation indicated the released substance is similar to atriopeptin III.
    • No evidence of atriopeptigen release was found.

    Conclusions:

    • The heart releases processed, low molecular weight atrial peptides (atriopeptins), not the high molecular weight precursor (atriopeptigen).
    • Atriopeptigen is likely proteolytically processed within the atria to form atriopeptins before release.
    • This suggests a specific processing pathway for atrial peptides before their secretion into circulation.