Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Correlations between phospholipid methylation and neuronal catecholamine transport.

M K Samet, C O Rutledge

    Biochemical Pharmacology
    |November 15, 1984
    PubMed
    Summary

    Inhibiting phospholipid methylation in neuronal membranes reduces norepinephrine uptake by decreasing transport efficiency. This suggests a link between membrane fluidity and neurotransmitter removal, impacting synaptic function.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Modulation of adrenergic neurotransmission in the rat tail artery by dietary lipids.

    Journal of cardiovascular pharmacology·1989
    Same author

    Inhibition of norepinephrine transport into synaptic vesicles by amphetamine analogs.

    The Journal of pharmacology and experimental therapeutics·1988
    Same author

    [3H]-myo-inositol uptake in rat cortical slices. Identification of Na+-dependent and Na+-independent systems.

    Biochemical pharmacology·1988
    Same author

    Effects of calcium depletion on norepinephrine- and A23187-induced stimulation of inositol phosphate formation.

    Biochemical pharmacology·1987
    Same author

    Effect of dietary lipids on myocardial norepinephrine content and field stimulation-mediated release of norepinephrine from perfused neonatal and adult rat hearts.

    Journal of cardiovascular pharmacology·1987
    Same author

    Comparison of norepinephrine- and veratrine-induced phosphoinositide hydrolysis in rat brain.

    The Journal of pharmacology and experimental therapeutics·1987

    Area of Science:

    • Neuroscience
    • Biochemistry
    • Cell Biology

    Background:

    • Biological membrane fluidity is influenced by phospholipid methylation.
    • Membrane properties can affect the function of transport proteins.
    • Norepinephrine (NE) uptake is crucial for synaptic function.

    Purpose of the Study:

    • To investigate the relationship between phospholipid methylation and norepinephrine uptake in rat cortical synaptosomes.
    • To determine the effect of inhibiting methylation on NE transport kinetics.

    Main Methods:

    • Used S-adenosylhomocysteine (AdoHcy) to inhibit phospholipid methylation.
    • Measured [3H]NE uptake in synaptosomes.
    • Performed kinetic analysis (Km, Vmax) of NE uptake.
    • Utilized adenosine, L-homocysteine thiolactone (HTL), and EHNA to induce intracellular AdoHcy.

    Related Experiment Videos

    Main Results:

    • AdoHcy decreased NE uptake in a concentration-dependent manner.
    • Inhibition of methylation correlated with reduced S-adenosylmethionine (AdoMet)-dependent phospholipid transmethylation.
    • Kinetic analysis showed decreased apparent Km and Vmax for NE uptake with AdoHcy.
    • Inducing intracellular AdoHcy decreased Vmax but not Km for NE uptake.

    Conclusions:

    • Enzymatic methylation of membrane phospholipids and norepinephrine transport are associated in neuronal membranes.
    • Inhibiting phospholipid methylation reduces neurotransmitter removal efficiency.
    • Altered membrane methylation may indirectly affect synaptic function.