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[Radioimmunoassay of 125I-sagamicin (author's transl)]
A radioimmunoassay for an aminoglycoside antibiotic, sagamicin, was developed using antisera from rabbits injected with a sagamicin-bovine serum albumin conjugate. Sagamicin was iodinated by a modified Bolton & Hunter method. The standard curve was linear on a logit-log plot yielding an sensitivity of 0.5ng/tube. A correlation coefficient of 0.95 was obtained between the radioimmunoassay and a microbioassay for sagamicin in human sera. Cross-reaction occurred with gentamicin components and some of sagamicin subunits, but there was no cross-reactivity to neomycin, kanamycin and amikacin. Antibody was purified by affinity chromatography on sagamicin bound agarose.
A radioimmunoassay for an aminoglycoside antibiotic, sagamicin, was developed using antisera from rabbits injected with a sagamicin-bovine serum albumin conjugate. Sagamicin was iodinated by a modified Bolton & Hunter method. The standard curve was linear on a logit-log plot yielding an sensitivity of 0.5ng/tube. A correlation coefficient of 0.95 was obtained between the radioimmunoassay and a microbioassay for sagamicin in human sera. Cross-reaction occurred with gentamicin components and some of sagamicin subunits, but there was no cross-reactivity to neomycin, kanamycin and amikacin. Antibody was purified by affinity chromatography on sagamicin bound agarose.