Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Modifications induced by ascorbic acid on alkaline phosphatase fluorescence.

G E Martorana, E Meucci, G A Miggiano

    The Italian Journal of Biochemistry
    |September 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Comparative species distribution model framework for marine conservation and its application to loggerhead turtles in the Mediterranean.

    Conservation biology : the journal of the Society for Conservation Biology·2026
    Same author

    Spatio-temporal dynamics of floating marine macro-litter in Italian waters (2018-2023): The impact of the COVID-19 pandemic.

    Environmental pollution (Barking, Essex : 1987)·2026
    Same author

    From juveniles to adults: Ontogenetic and seasonal patterns of loggerhead turtles in oceanic Mediterranean habitats.

    Marine environmental research·2026
    Same author

    Non-thyroidal illness syndrome in chronic diseases: role of irisin as modulator of antioxidants.

    European review for medical and pharmacological sciences·2023
    Same author

    Vespa velutina nigrithorax venom allergy: inhibition studies approach for the choice of specific immunotherapy.

    European annals of allergy and clinical immunology·2022
    Same author

    Review of decontamination protocols for shared non-critical objects in 35 policies of UK NHS acute care organizations.

    The Journal of hospital infection·2021
    Same journal

    Alpha-bisabolol: unexpected plant-derived weapon in the struggle against tumour survival?

    The Italian journal of biochemistry·2009
    Same journal

    Expression of succinate dehydrogenase flavoprotein subunit in saccharomyces cerevisiae studied by lacZ reporter strategy. Effect of FLX1 deletion.

    The Italian journal of biochemistry·2009
    Same journal

    The smaller isoform of the mitochondrial transcription factor A has a role in the mitochondrial transcription.

    The Italian journal of biochemistry·2009
    Same journal

    Functional proteomics: protein-protein interactions in vivo.

    The Italian journal of biochemistry·2009
    Same journal

    Synergistic effect of Ca2+ and peroxidized cardiolipin in the induction of permeability transition and cytochrome c release in rat heart mitochondria.

    The Italian journal of biochemistry·2009
    Same journal

    Neurosteroid-growth factor cross-talk induces up and down regulation of GFAP and vimentin expression in serum free astrocyte cultures.

    The Italian journal of biochemistry·2009
    See all related articles

    Ascorbic acid and its derivatives quench alkaline phosphatase fluorescence, indicating protein-specific interactions. These findings offer insights into inhibitor-enzyme dynamics and transition phenomena.

    Area of Science:

    • Biochemistry
    • Enzymology
    • Biophysics

    Background:

    • Alkaline phosphatase is a crucial enzyme with various physiological roles.
    • Ascorbic acid, isoascorbic acid, and dehydroascorbic acid are related compounds with potential biological activities.

    Purpose of the Study:

    • To investigate the interaction between ascorbic acid, isoascorbic acid, dehydroascorbic acid, and alkaline phosphatase.
    • To explore the quenching of tryptophyl fluorescence in alkaline phosphatase by these compounds.
    • To understand the mechanism and implications of inhibitor-enzyme interactions.

    Main Methods:

    • Spectroscopic analysis of tryptophyl fluorescence quenching.
    • Enzyme kinetics to assess inhibitory efficiency.
    • Investigation of inactivation and emission deactivation patterns.

    Related Experiment Videos

    Main Results:

    • Ascorbic acid, isoascorbic acid, and dehydroascorbic acid effectively quench alkaline phosphatase tryptophyl fluorescence.
    • The quenching is protein-aspecific but correlates with the inhibitory efficiency of each compound.
    • Observed similarities in kinetic inactivation and emission deactivation suggest a transition phenomenon.

    Conclusions:

    • The ascorbic acid system provides a valuable tool for studying inhibitor-enzyme interactions.
    • Fluorescence quenching reveals insights into the transition phenomena during enzyme inhibition.
    • Understanding these interactions is crucial for enzyme mechanism studies.