Determination of bromopride in human plasma and urine by high-performance liquid chromatography

Bromopride was measured in plasma and urine using reversed-phase high-performance liquid chromatography employing ultraviolet absorption detection. The limit of detection in plasma was 2 ng/ml, sufficient for pharmacokinetic studies of the drug. Plasma concentrations of bromopride reached mean peak levels (55 ng/ml) at 1 h after single oral doses of 20 mg and declined with a half-life of 4.9 h. Less than a mean of 10% of an oral dose was excreted unchanged in the urine. The assay could also be used to measure metoclopramide in these bio-fluids.