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A new method for sister-chromatid exchange studies in vivo, a single injection of BrdU in melted agar
Mutation Research
|November 1, 1984
Abstract:
Details are given for a new, rapid, and simple method of BrdU administration for SCE studies in vivo. BrdU was dissolved in melted agar and administered to mice as a single subcutaneous injection. Clear and reproducible sister chromatid differential staining of bone marrow cells was obtained. Melted agar, when injected into mice, solidified quickly and formed a clump which provided a sustained release of BrdU. HPLC analyses showed that a considerable serum level of BrdU was maintained for at least 10 h in a mouse injected with 25 mg of BrdU in agar.

