Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Stable tRNA precursors in HeLa cells.

F Harada, M Matsubara, N Kato

    Nucleic Acids Research
    |December 21, 1984
    PubMed
    Summary

    Researchers sequenced two transfer RNA (tRNA) precursors from human cells, identifying extra nucleotides and undermodified nucleosides. These findings offer insights into tRNA maturation and stability in higher eukaryotes.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Novel Method to Target Apical Periodontitis Worsened by Inflammatory Bowel Disease.

    Journal of dental research·2026
    Same author

    A neck-preserving short stem better reconstructs the centre of rotation than straight stems: a computed tomography-based cadaver study.

    Archives of orthopaedic and trauma surgery·2021
    Same author

    Amino acid charge and epidermolysis bullosa simplex severity: genotype-phenotype correlations.

    Journal of the European Academy of Dermatology and Venereology : JEADV·2019
    Same author

    Molecular alterations of newly formed mandibular bone caused by zoledronate.

    International journal of oral and maxillofacial surgery·2018
    Same author

    A new evaluation of masticatory ability in patients with dysphagia: The Saku-Saku Test.

    Archives of gerontology and geriatrics·2017
    Same author

    Pleomorphic lobular carcinoma of the breast: a comparison of cytopathological features with other lobular carcinoma variants.

    Cytopathology : official journal of the British Society for Clinical Cytology·2016

    Area of Science:

    • Molecular Biology
    • Eukaryotic Gene Expression
    • RNA Biochemistry

    Background:

    • Transfer RNA (tRNA) molecules are essential for protein synthesis, acting as adaptors between mRNA codons and amino acids.
    • The processing and maturation of tRNA precursors involve complex biochemical pathways, particularly in higher eukaryotes.
    • Understanding tRNA precursor structures is crucial for elucidating gene expression regulation.

    Purpose of the Study:

    • To determine the nucleotide sequences of naturally occurring tRNA precursors from higher eukaryotes.
    • To characterize the structural features and modifications of these tRNA precursors.
    • To investigate the potential role of structural elements in tRNA precursor stability.

    Main Methods:

    • Isolation of tRNA precursors from HeLa cells using two-dimensional polyacrylamide gel electrophoresis.
    • Sequencing of isolated tRNA precursors.
    • Analysis of nucleotide composition and secondary structure.

    Main Results:

    • Sequencing of two tRNA precursors, tRNAMet and tRNALeu, from HeLa cells.
    • Identification of extra nucleotides at both the 5' (including 5'-triphosphates) and 3' (including oligo U) ends of the precursors.
    • Detection of undermodified nucleosides (e.g., m2G, t6A, ac4C) compared to mature tRNAs.
    • Observation of two additional hydrogen bonds in the clover leaf structures of the precursors.

    Conclusions:

    • These are the first determined sequences of naturally occurring tRNA precursors from higher eukaryotes.
    • The extra nucleotides and undermodified nucleosides suggest specific processing steps.
    • Additional hydrogen bonds in precursor structures may contribute to their stability before maturation.

    Related Experiment Videos