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[Characteristics of Pseudomonas putida plasmid DNAs]
Mikrobiologiia
|January 1, 1978
Summary
Pseudomonas putida G7 contains multiple plasmid DNAs involved in biodegradation. These plasmids, with varying molecular weights, are primarily associated with the bacterium
Area of Science:
- Microbiology
- Molecular Biology
- Biochemistry
Background:
- Pseudomonas putida G7 is a bacterium known for its metabolic versatility.
- Plasmids are extrachromosomal DNA molecules that can confer advantageous traits to host bacteria.
- Understanding plasmid characteristics is crucial for microbial genetics and biotechnology.
Purpose of the Study:
- To investigate the physico-chemical properties of plasmid DNAs from Pseudomonas putida G7.
- To analyze the behavior of these plasmid DNAs during chromatographic separation.
- To determine the molecular weights and localization of identified plasmid DNAs.
Main Methods:
- Isolation and purification of plasmid DNAs from Pseudomonas putida G7.
- Chromatography using Sepharose 4B and Kieselguhr with methylated albumin (MAC).
- Determination of molecular weights of isolated plasmid DNAs.
Main Results:
- Several distinct plasmid DNAs were identified in Pseudomonas putida G7.
- The molecular weights of these plasmids ranged from 3-5x10^6, 15-18x10^6, and 33-36x10^6 daltons.
- Chromatographic behavior indicated specific interactions with the separation matrices.
Conclusions:
- Pseudomonas putida G7 harbors multiple plasmids with significant variations in size.
- The study suggests that biodegradation-related plasmid DNAs are predominantly located near the bacterial chromosomes.
- A minor fraction of these plasmids exhibits extrachromosomal localization, impacting their stability and inheritance.