Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human testicular cell suspensions in vitro using post mortem material.

A J Dietrich

    Andrologia
    |November 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Primary care pediatricians' roles and perceived responsibilities in the identification and management of depression in children and adolescents.

    Ambulatory pediatrics : the official journal of the Ambulatory Pediatric Association·2002
    Same author

    Identifying physicians likely to benefit from depression education: a challenge for health care organizations.

    Medical care·2001
    Same author

    An encounter-based analysis of the nature of teaching and learning in a 3rd-year medical school clerkship.

    Teaching and learning in medicine·2001
    Same author

    The telephone as a new weapon in the battle against depression.

    Effective clinical practice : ECP·2001
    Same author

    Persistent increase in children's sun protection in a randomized controlled community trial.

    Preventive medicine·2000
    Same author

    Differences in ambulatory teaching and learning by gender match of preceptors and students.

    Family medicine·2000
    Same journal

    Correction.

    Andrologia·2023
    Same journal

    The combination of ginger and zinc supplement could improve lead-induced reproductive dysfunction by inhibiting apoptosis mediated by oxidative damage and inflammation.

    Andrologia·2022
    Same journal

    Testosterone and lifespan in males: At the right time and the right level.

    Andrologia·2022
    Same journal

    CORRIGENDUM.

    Andrologia·2022
    Same journal

    CORRIGENDUM.

    Andrologia·2022
    Same journal

    Microfluidic devices employing chemo- and thermotaxis for sperm selection can improve sperm parameters and function in patients with high DNA fragmentation.

    Andrologia·2022
    See all related articles

    This study presents a novel method for culturing human testicular cells from post-mortem tissue for over 10 days. The technique ensures cell survival and metabolic coupling through undisturbed cell layers and continuous perfusion in serum-free media.

    Area of Science:

    • Reproductive biology
    • Cell culture techniques
    • Post-mortem tissue preservation

    Background:

    • Human testicular cells are crucial for reproductive research.
    • Maintaining viability of post-mortem cells for extended periods is challenging.
    • Current methods often require serum-containing media, limiting certain research applications.

    Purpose of the Study:

    • To develop a reliable method for long-term culturing of human testicular cells from post-mortem samples.
    • To establish a serum-free culture system for enhanced cell viability and function.
    • To demonstrate the active survival and intercellular communication of cultured cells.

    Main Methods:

    • Utilizing a perfusion-based system for continuous medium renewal.
    • Culturing human testicular cells in a serum-free medium.

    Related Experiment Videos

  • Maintaining an undisturbed layer of testicular cells on the culture flask surface.

    • Main Results:

    • Successful culturing of human testicular cells for at least 10 days.
    • Demonstration of undisturbed cell layer integrity throughout the culture period.
    • Observation of cell junction formation and metabolic coupling within hours, indicating active survival.

    Conclusions:

    • The described perfusion method enables successful long-term culturing of post-mortem human testicular cells.
    • Serum-free conditions combined with perfusion support cell viability and intercellular communication.
    • This technique offers a valuable tool for studying testicular cell biology and function using post-mortem material.