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Related Experiment Videos

Histamine uptake by leukocytes in vitro.

C Catini, G Gheri, M Giampaoli

    Basic and Applied Histochemistry
    |January 1, 1984
    PubMed
    Summary

    Human leukocytes efficiently uptake exogenous histamine, with neutrophils and eosinophils showing the highest capacity. Unexpectedly, basophils demonstrated a poor capacity for histamine uptake in this study.

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    Histology and histopathology·2004

    Area of Science:

    • Immunology
    • Cell Biology
    • Pharmacology

    Background:

    • Leukocytes play a crucial role in immune responses.
    • Histamine is a key mediator in allergic and inflammatory reactions.
    • Understanding histamine-leukocyte interactions is vital for immune system research.

    Purpose of the Study:

    • To investigate the capacity of human leukocytes to uptake exogenous histamine.
    • To determine the differential uptake of histamine among various leukocyte subtypes.

    Main Methods:

    • Human leukocytes were incubated with varying concentrations of histamine (0-100 µg/ml) at 37.5°C for 1 hour.
    • Histamine uptake was quantified using a photofluorometric assay.
    • Intracellular histamine concentrations were measured relative to control levels.

    Main Results:

    • Leukocytes demonstrated significant uptake of exogenous histamine, with intracellular concentrations increasing substantially above controls.
    • Neutrophils and eosinophils exhibited the highest histamine uptake capabilities.
    • Basophils showed a notably lower capacity for histamine uptake than anticipated.

    Conclusions:

    • Human leukocytes, particularly neutrophils and eosinophils, actively internalize exogenous histamine.
    • The differential uptake capacity among leukocyte types, especially the low uptake by basophils, warrants further investigation.
    • These findings contribute to understanding histamine's role in leukocyte function and immune modulation.

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