Linkage of diabetes insipidus and agouti genes in the rat

Linkage of the hooded (h), agouti (A), and diabetes insipidus (di) genes was found in (ACI X DI)F1 X DI backcross rats. The genetic map distance A-di for females and for males was 19 +/- 5 and 28 +/- 5 cM, respectively. However, this difference was not significant. The combined data showed the map distance to be 25 +/- 4 cM. The three-point cross showed the following corrected distances and order of genes: h-42 +/- 4-A-25 +/- 4-di. However, the linkage of h and A, although significant (chi 2 = 9.03, P less than 0.001), is only tentative and must be confirmed by additional studies.