Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Globin-methionine complexes formed during labelling studies.

E Beutler, T Gelbart

    Clinical and Laboratory Haematology
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    1342C mutation in Gaucher's disease.

    Lancet (London, England)·1995
    Same author

    Triosephosphate isomerase deficiency: repetitive occurrence of point mutation in amino acid 104 in multiple apparently unrelated families.

    American journal of hematology·1995
    Same author

    Gaucher disease.

    Medicine·1995
    Same author

    Treatment regimens in Gaucher's disease.

    Lancet (London, England)·1995
    Same author

    Molecular study of pyruvate kinase deficient patients with hereditary nonspherocytic hemolytic anemia.

    The Journal of clinical investigation·1995
    Same author

    Erythrocyte fragility and chronic intermittent pigmenturia in a dog.

    Journal of the American Veterinary Medical Association·1995
    Same journal

    Cystic fibrosis presenting with haematological abnormalities.

    Clinical and laboratory haematology·2006
    Same journal

    T-cell-rich B-cell lymphoma of the spleen presenting with severe hypersplenism.

    Clinical and laboratory haematology·2006
    Same journal

    Spontaneous splenic haematoma in a multiple myeloma patient receiving pegfilgrastim support.

    Clinical and laboratory haematology·2006
    Same journal

    Analysis of the CDR3 region of alpha/beta T-cell receptors (TCRs) and TCR BD gene double-stranded recombination signal sequence breaks end in peripheral blood mononuclear cells of T-lineage acute lymphoblastic leukemia.

    Clinical and laboratory haematology·2006
    Same journal

    Characterization of several amino acid transports and glutamine metabolism in MOLT4 human T4 leukemia cells.

    Clinical and laboratory haematology·2006
    Same journal

    Osteomylelitis and necrosis of the jaw in patients treated with bisphosphonates: a comparative study focused on multiple myeloma.

    Clinical and laboratory haematology·2006
    See all related articles

    Aberrant results in globin synthesis studies using 35S-methionine labeling were traced to methionine binding to globin chains. This binding can be prevented by adding specific amino acids during protein synthesis.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Protein Synthesis

    Background:

    • Cellogel separation is a technique used for globin chain analysis.
    • 35S-methionine is commonly used to label newly synthesized proteins.

    Purpose of the Study:

    • To investigate the cause of aberrant results encountered during globin synthetic measurements using 35S-methionine.
    • To identify the nature of the prominent radioactive bands observed trailing alpha and beta globin chains.

    Main Methods:

    • Cellogel electrophoresis of 35S-methionine-labelled globin chains.
    • Comparison with 3H-leucine labeling.
    • Testing the effect of cystine, cysteine, and homocysteine on band formation.
    • Assessing the removal of bands by precipitation and dialysis.

    Related Experiment Videos

    Main Results:

    • Prominent radioactive bands trailing alpha and beta globin chains were observed with 35S-methionine, but not with 3H-leucine.
    • These bands were prevented by cystine, cysteine, or homocysteine.
    • Bands were resistant to precipitation but removable by urea dialysis.
    • The bands represent firm, non-covalent binding of methionine to globin during synthesis.

    Conclusions:

    • The study identifies a non-covalent binding of methionine to globin chains as a source of artifact in protein synthesis studies.
    • This methionine binding phenomenon may impact the study of other protein syntheses beyond globin.
    • Understanding this artifact is crucial for accurate interpretation of protein labeling experiments.