Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Structure of DNA complexes with regular polypeptides].

A S Krylov, G V Gurskiĭ, N O Kondrat'eva

    Molekuliarnaia Biologiia
    |March 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Differential Diagnosis of Specific Cardiomyopathy in HIV-Adult With Myocardial Perfusion Imaging Via Single Photon Emission Computed Tomography].

    Kardiologiia·2025
    Same author

    [Methodical approaches to annotation and labeling of histological images in order to automatically detect the layers of the stomach wall and the depth of invasion of gastric cancer].

    Arkhiv patologii·2022
    Same author

    High-pressure transformation of dithiazolylidene-dithiazolium polyiodide with N-H…N hydrogen bond: A Raman Spectroscopy study.

    Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2021
    Same author

    [Severe spine lesion following alkaptonuria. Case report].

    Zhurnal voprosy neirokhirurgii imeni N. N. Burdenko·2021
    Same author

    Spectroscopy of structurally disordered hydrated iron fluoridotitanate in the regions of vibrational and electronic excitations.

    Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2021
    Same author

    Synthesis of Samarium OxysulfateSm<sub>2</sub>O<sub>2</sub>SO<sub>4</sub> in the High-Temperature Oxidation Reaction and Its Structural, Thermal and Luminescent Properties.

    Molecules (Basel, Switzerland)·2020
    Same journal

    [microRNA in the Realization of Molecular Mechanisms of Lymphocyte Development and Their Malignant Transformation].

    Molekuliarnaia biologiia·2026
    Same journal

    [Glial Progenitor Cell Therapy Improves Mitochondrial Function in the Hippocampus of 5xFAD Mice, but Does Not Restore the Multiscale Structure of Behavioral Stress Response].

    Molekuliarnaia biologiia·2026
    Same journal

    [Organ-Specific Changes in the Expression of Inflammatory Response and Antioxidant Defense Genes in the Late Period after Hemorrhagic Shock].

    Molekuliarnaia biologiia·2026
    Same journal

    [GLUT8- and AMPK-Dependent Autophagy Signaling in the Mechanism of the Neuroprotective Action of Trehalose].

    Molekuliarnaia biologiia·2026
    Same journal

    [Biochip for Determination of 92 Human Y-Chromosome Haplogroups by SNP Markers and Frequency Distribution of These Haplogroups in Slavic Population of European Russia].

    Molekuliarnaia biologiia·2026
    Same journal

    [The Level of Methylation in the Promoter Regions of DNA Repair Genes in Chronically Exposed People].

    Molekuliarnaia biologiia·2026
    See all related articles

    This study investigated polypeptide-DNA interactions using circular dichroism (CD). Polypeptide binding to DNA was sensitive to environmental changes, suggesting structural relaxation and liquid-crystal domains.

    Area of Science:

    • Biochemistry
    • Biophysics
    • Molecular Biology

    Context:

    • Investigating the structural properties of synthetic polypeptides.
    • Understanding the complex formation between polypeptides and DNA.
    • Utilizing biophysical techniques like circular dichroism (CD) and ethidium bromide binding assays.

    Purpose:

    • To study the conformation of various regular polypeptides.
    • To characterize the binding of these polypeptides to DNA.
    • To determine the extent of DNA coverage and the length of DNA occupied by polypeptides.

    Summary:

    • Circular dichroism (CD) spectroscopy was used to analyze the conformation of synthetic polypeptides, including (Lys-Ala)50, (Lys-Ala2)37, (Lys-Ala2)26, (Lys-Ala3)18, (Lys3-Pro)29, and (Orn3-Gly)28.
    • Polypeptide-DNA complexes were formed via jump-dilution, and their binding characteristics were assessed using ethidium bromide binding isotherms and energy transfer measurements to estimate DNA coverage and bound length.

    Related Experiment Videos

  • The CD spectra of these complexes exhibited high sensitivity to environmental factors such as temperature and ionic strength, indicating dynamic structural changes.

    • Impact:

    • The findings suggest that polypeptide-DNA complexes undergo structural relaxation and may form liquid-crystal domains in solution.
    • This research provides insights into the molecular mechanisms of DNA-polypeptide interactions.
    • Understanding these interactions is crucial for fields ranging from gene regulation to drug delivery systems.