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A method for preparation of dry thrombin for topical application
Cryobiology
|December 1, 1984
Summary
Bovine and human plasma can yield topical hemostasis thrombin. This study details isolating prothrombin, activating it, and purifying thrombin using DEAE-Sephadex A-50 and acetone, achieving high specific activity.
Area of Science:
- Biochemistry
- Biotechnology
- Hematology
Background:
- Thrombin is crucial for topical hemostasis.
- Effective methods for preparing thrombin from plasma are essential for medical applications.
Purpose of the Study:
- To describe a method for isolating and activating prothrombin from plasma.
- To purify thrombin for topical hemostasis.
- To quantify the specific activity and yield of the prepared thrombin.
Main Methods:
- Prothrombin isolation using DEAE-Sephadex A-50 adsorption.
- Prothrombin activation using calcium chloride (CaCl2) and thromboplastin.
- Thrombin precipitation and purification utilizing acetone.
Main Results:
- The thrombin preparation achieved a specific activity of 122 ± 23 IU/mg protein.
- The overall yield was 36,360 ± 6623 IU/liter of plasma.
- The method allows for the preparation of thrombin from both bovine and human plasma.
Conclusions:
- A reproducible method for preparing topical hemostasis thrombin from plasma has been established.
- The characterized specific activity and yield indicate the efficacy of the purification process.
- This preparation method holds potential for clinical use in hemostasis.