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A high selective screening test for methamphetamine in human urine.

Y Nakahara, H Sekine

    Forensic Science International
    |December 1, 1984
    PubMed
    Summary
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    A new Ad-Tip screening test accurately detects methamphetamine in urine within 3 minutes. This rapid method shows results comparable to laboratory analyses for drug testing.

    Area of Science:

    • Forensic Toxicology
    • Analytical Chemistry
    • Clinical Chemistry

    Background:

    • Methamphetamine is a widely abused stimulant.
    • Accurate and rapid drug screening tests are crucial for clinical and forensic settings.
    • Existing screening methods may lack speed or selectivity.

    Purpose of the Study:

    • To develop a highly selective and rapid screening test for methamphetamine in human urine.
    • To evaluate the efficiency and accuracy of the Ad-Tip method.

    Main Methods:

    • Utilized an Ad-Tip (ODS-silica minicolumn) for sample extraction.
    • Employed a washing step, elution with modified Simon's reagent, and coloration with carbonate buffer.
    • Determined the detection limit and assay time per sample.

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    Main Results:

    • Achieved a detection limit of 1 microgram/ml for methamphetamine in urine.
    • Completed each test within 3 minutes per sample.
    • Demonstrated near-identical results to established laboratory analyses.

    Conclusions:

    • The Ad-Tip method provides a rapid and highly selective screening tool for methamphetamine.
    • This method offers a viable alternative for quick and accurate urine drug testing.
    • The test's speed and accuracy make it suitable for high-throughput screening.