Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Allogeneic reactivity in normal mouse serum.

M A Lynes, L Flaherty, J Michaelson

    Journal of Immunogenetics
    |June 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    KatG catalase deficiency confers bedaquiline hyper-susceptibility to isoniazid resistant <i>Mycobacterium tuberculosis</i>.

    bioRxiv : the preprint server for biology·2023
    Same author

    Controlled Continuous Evolution of Enzymatic Activity Screened at Ultrahigh Throughput Using Drop-Based Microfluidics.

    Angewandte Chemie (International ed. in English)·2023
    Same author

    Evaluation of potential health effects associated with occupational and environmental exposure to styrene - an update.

    Journal of toxicology and environmental health. Part B, Critical reviews·2019
    Same author

    Severe maternal morbidity during delivery hospitalisation in a large international administrative database, 2008-2013: a retrospective cohort.

    BJOG : an international journal of obstetrics and gynaecology·2019
    Same author

    Challenges and Opportunities for Occupational Epidemiology in the Twenty-first Century.

    Current environmental health reports·2017
    Same author

    Mortality risk among workers with exposure to dioxins.

    Occupational medicine (Oxford, England)·2016
    Same journal

    VHDJH to JH joining is not blocked in mu-chain-producing pre-B cells, implying the breakdown of allelic exclusion.

    Journal of immunogenetics·1990
    Same journal

    Characterization of soluble HLA molecules in sweat and quantitative HLA differences in serum of healthy individuals.

    Journal of immunogenetics·1990
    Same journal

    Characterization of MHC ancestral haplotypes associated with insulin-dependent diabetes mellitus: evidence for involvement of non-HLA genes.

    Journal of immunogenetics·1990
    Same journal

    Increased interleukin-2 receptor affinity in normal HLA A1 B8 DR3 subjects.

    Journal of immunogenetics·1990
    Same journal

    Immunogenetic aspects of human thyroglobulin-reactive T cell lines and hybridomas.

    Journal of immunogenetics·1990
    Same journal

    Evidence against extended DR3-related haplotypes in Graves' disease.

    Journal of immunogenetics·1990
    See all related articles

    Normal mouse serum shows specific reactivity against certain stimulated mouse spleen cells, particularly those expressing H-2Kk antigens. This suggests immune responses may be influenced by environmental factors or internal controls.

    Area of Science:

    • Immunology
    • Histocompatibility
    • Cellular Immunology

    Background:

    • Normal C57BL/6 (B6) mouse serum was analyzed for specific reactivity.
    • Reactivity was assessed against lipopolysaccharide (LPS)-stimulated mouse spleen cells.

    Purpose of the Study:

    • To investigate specific immune reactivity in normal mouse serum.
    • To identify antigens targeted by serum antibodies in LPS-stimulated cells.

    Main Methods:

    • Direct cytotoxicity assay was employed.
    • Reactivity was tested against LPS-stimulated spleen cells from various mouse strains.

    Main Results:

    • Selective serum reactivity was observed against lymphoblasts expressing H-2Kk antigens.

    Related Experiment Videos

  • Congenic mouse strains at the major histocompatibility complex (MHC) did not show similar alloreactivity.
  • Serum from a congenic strain differing at Tla and nu loci also showed reactivity, indicating T cell independence.

    • Conclusions:

    • Normal mouse serum can exhibit specific reactivity against H-2Kk antigen-expressing cells.
    • This reactivity is independent of a competent T cell compartment.
    • Potential origins include environmental stimulation, internal immunoregulatory controls, or a combination thereof.