[Effect of changes in the pH of the medium on neuroblastoma cell differentiation in culture]

Morphological differentiation of neuroblastoma cells in culture was found to be depended on extracellular pH value. Increase of pH value up to 8.2 resulted in the appearance of both differentiated and dividing cell populations. The morphologically mature state of differentiated cells was observed at 7th-8th day and their population remained stable state for over 4 weeks. Increase of pH value to 8.5-8.9 caused retraction of cell processes of differentiated cells and inhibited multiplication of the dividing cells.