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Related Experiment Videos

Mineralization of developing teeth.

M B Engel, O H Hilding

    Scanning Electron Microscopy
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    This study reveals essential element distribution during mouse molar development. Calcium and phosphorus gradients form in enamel and dentine matrices, influencing mineralization.

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    Comment on mandibular growth.

    American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics·1992

    Area of Science:

    • Biochemistry
    • Developmental Biology
    • Materials Science

    Background:

    • Tooth mineralization involves complex elemental distribution within developing enamel and dentine.
    • Understanding these elemental gradients is crucial for comprehending tooth formation and integrity.

    Purpose of the Study:

    • To investigate the distribution of essential elements, particularly calcium (Ca) and phosphorus (P), in developing mouse molar matrices and cellular layers.
    • To elucidate the role of these elements and their ratios in the process of biomineralization.

    Main Methods:

    • Electron probe analysis was employed to quantify elemental concentrations.
    • Polarization microscopy supplemented the elemental analysis to study matrix properties.

    Main Results:

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    • Distinct distribution gradients for Ca, P, and Ca/P ratios were observed in enamel, dentine, and predentine matrices.
    • Low Ca/P ratios in early mineralization suggest amorphous calcium phosphate or protein-bound calcium.
    • Elevated calcium concentrations were noted in odontoblast and ameloblast layers, peaking in ameloblasts at 14 days.
    • Sulfur concentration significantly decreases from early enamel to mature enamel, and from predentine to dentine, linked to changes in organic components like methionine and sulfated glycosaminoglycans.

    Conclusions:

    • Mineralization is associated with water loss, compositional changes, and altered aggregation states within the negatively charged matrices.
    • The accumulation of calcium in various forms during mineralization can be explained by Gibbs-Donnan equilibrium principles.