Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The mesothelial cell as a non-thrombogenic surface.

L J Nicholson, J M Clarke, R M Pittilo

    Thrombosis and Haemostasis
    |October 31, 1984
    PubMed
    Summary

    Researchers developed a method to harvest mesothelial cells from omentum tissue. These cultured cells showed a non-thrombogenic surface, supporting blood flow without platelet adhesion in vitro.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Black Point Reaction of Durum and Common Wheat Cultivars Grown Under Irrigation in Southern Saskatchewan.

    Plant disease·2019
    Same author

    Subacute cutaneous lupus erythematosus and dermatomyositis associated with anti-programmed cell death 1 therapy.

    The British journal of dermatology·2018
    Same author

    Pilot feasibility study examining a structured self-management diabetes education programme, DESMOND-ID, targeting HbA<sub>1c</sub> in adults with intellectual disabilities.

    Diabetic medicine : a journal of the British Diabetic Association·2017
    Same author

    Targeting tumor multicellular aggregation through IGPR-1 inhibits colon cancer growth and improves chemotherapy.

    Oncogenesis·2017
    Same author

    Understanding the mechanisms of action of antiangiogenic agents in metastatic colorectal cancer: a clinician's perspective.

    Cancer treatment reviews·2014
    Same author

    Genetics of pre-harvest sprouting resistance in a cross of Canadian adapted durum wheat genotypes.

    Molecular breeding : new strategies in plant improvement·2014

    Area of Science:

    • Cell Biology
    • Tissue Engineering
    • Biomedical Science

    Background:

    • Mesothelial cells line serous cavities and play roles in lubrication and immune response.
    • Harvesting and culturing mesothelial cells is crucial for research and potential therapeutic applications.
    • Previous methods for obtaining mesothelial cells may be limited in efficiency or applicability.

    Purpose of the Study:

    • To describe and validate a novel technique for harvesting and culturing mesothelial cells.
    • To assess the biocompatibility and hemocompatibility of cultured mesothelial cells.
    • To investigate the potential of these cells in supporting blood flow.

    Main Methods:

    • Omentum tissue was subjected to collagenase digestion to isolate mesothelial cells.

    Related Experiment Videos

  • Cells were cultured and characterized using scanning electron microscopy.
  • An in vitro system (Baumgartner chamber) exposed cultured cells to blood.

    • Main Results:

    • The collagenase digestion technique successfully yielded viable mesothelial cells from both rat and human omentum.
    • Scanning electron microscopy confirmed the morphology of cultured rat mesothelial cells.
    • Cultured mesothelial cells supported blood flow in vitro and prevented platelet adhesion, unlike control fibroblasts.

    Conclusions:

    • A reliable method for harvesting and culturing mesothelial cells from omentum has been established.
    • Cultured mesothelial cells exhibit non-thrombogenic properties, indicating potential for vascular applications.
    • This technique provides a valuable tool for studying mesothelial cell function and exploring their therapeutic use.