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[Effect of iron on Pasteurella multocida].

K D Flossmann, G Müller, P Heilmann

    Zentralblatt Fur Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology
    |October 1, 1984
    PubMed
    Summary

    Pasteurella multocida requires iron for growth and virulence. This bacterium possesses an unknown iron transport system capable of utilizing various iron sources, but not all iron compounds are accessible.

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    Area of Science:

    • Microbiology
    • Bacterial Pathogenesis
    • Iron Metabolism

    Background:

    • Iron is crucial for the growth, virulence, and immunogenicity of Pasteurella multocida.
    • Previous studies in vitro and in vivo (mice, piglets, calves) have highlighted iron's importance.
    • The specific iron uptake mechanisms of P. multocida remain largely uncharacterized.

    Purpose of the Study:

    • To investigate the iron transport system of Pasteurella multocida.
    • To determine which iron sources are available for P. multocida growth and iron assimilation.
    • To assess the impact of various iron chelating agents on P. multocida.

    Main Methods:

    • In vitro experiments using bacterial cultures.
    • Radioactive iron (Fe-59) tracer studies to monitor iron uptake.
    • Testing the effects of diverse iron substrates and chelating agents on bacterial growth and iron incorporation.

    Main Results:

    • P. multocida can acquire iron from physiological sources like heme, ferritin, transferrin, and lactoferrin.
    • Iron from iron dextran and low molecular weight iron compounds is available to P. multocida.
    • Certain iron chelators (e.g., alpha,alpha'-dipyridyl, phenanthroline, deferrioxamine B, enterobactin) inhibit P. multocida growth and iron uptake, while others have minimal effect.

    Conclusions:

    • P. multocida possesses a versatile iron transport system capable of utilizing various iron sources.
    • Enterobactin and deferrioxamine B, common siderophores, do not appear to be involved in P. multocida iron transport.
    • Understanding P. multocida's iron acquisition is key to developing strategies against infections caused by this pathogen.

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