Interaction of vitamin A deficiency and excess fluoride in calcification of rat bones and teeth

Using a 2 X 2 factorial design, we evaluated the possible interaction of vitamin A deficiency and excess fluoride in rat dentin and bone. Simultaneous presence of excess fluoride plus vitamin A deficiency resulted in a significant decrease in bone fluoride concentration compared to the presence of excess fluoride alone. Vitamin A deficiency alone significantly reduced calcium concentration in dentin formed during the deficient period.