The effect of cyclophosphamide on artificial metastases formation in the lungs of Balb/c mice

Cyclophosphamide (CY) administration prior to intravenous tumor cells injection resulted in a rapid increase of the number of neoplastic nodules in the lungs. It was tested whether the elimination of the possible suppressive effect of CY on the immunological system by reconstitution of the animals with the spleen lymphocytes would diminish CY effect and reduce the number of tumor nodules in the lungs. This procedure only partially eliminates the effect of the growing number of artificial metastases.