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Identification of an hyperglycemic factor from heated serum
Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|January 1, 1984
Summary
In vitro heated serum induced hyperglycemia in rats. A specific molecular weight fraction (2,000-11,000 Da) likely contains a heat-induced polypeptide responsible for this effect.
Area of Science:
- Biochemistry
- Endocrinology
- Physiology
Background:
- Serum heating can alter its biochemical properties.
- Hyperglycemia is a critical metabolic state with various causes.
Purpose of the Study:
- To investigate the effects of in vitro heated serum on glucose metabolism in rats.
- To identify potential factors in heated serum that induce hyperglycemia.
Main Methods:
- Serum was heated in vitro (44°C for 15 minutes).
- Serum was fractionated using gel filtration and size exclusion chromatography.
- Fractions were injected into Wistar rats to assess glycemic response.
Main Results:
- A specific serum fraction induced hyperglycemia in rats.
- The hyperglycemic fraction had a molecular weight between 2,000 and 11,000 Daltons.
Conclusions:
- In vitro heat treatment of serum can generate a hyperglycemic factor.
- This factor is likely a polypeptide within the 2,000-11,000 Da molecular weight range.