Identification of an hyperglycemic factor from heated serum

A fraction of in vitro heated serum (44 degrees C, 15 minutes) obtained by gel filtration chromatography and high performance size exclusion chromatography was found to induce an hyperglycemia when injected to Wistar rats. This fraction corresponded to a molecular weight range of 2,000-11,000. We speculate that this fraction contained a polypeptide responsible of hyperglycemia and induced by hyperthermia.