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A human monoclonal antibody specific for tetanus toxoid
Developments in Biological Standardization
|January 1, 1984
Summary
Researchers successfully created hybrid cells by fusing lymphocytes with a human B lymphoma line. These hybrid cells demonstrated significant in vitro activity against tetanus toxoid, showing promise for tetanus immunity.
Area of Science:
- Immunology
- Cell Biology
- Hybridoma Technology
Background:
- Peripheral blood lymphocytes from tetanus toxoid-immunized donors were used.
- The human B lymphoma cell line RH-L-4 served as the fusion partner.
Purpose of the Study:
- To achieve successful hybridization between stimulated lymphocytes and a B lymphoma line.
- To evaluate the in vitro and in vivo efficacy of the resulting hybrid cells against tetanus toxoid.
Main Methods:
- Hybridization of peripheral blood lymphocytes with the RH-L-4 B lymphoma line.
- Selection and cloning of hybrid cells.
- In vitro assessment of anti-tetanus toxoid activity.
- Radioimmunoassay (RIA) and mouse protection tests for specific activity evaluation.
Main Results:
- Successful generation of hybrid cells through fusion.
- Selected hybrid clones exhibited significant in vitro activity against tetanus toxoid (1-5 µg/10^6 cells/24 hours).
- One hybrid clone showed comparable specific activity to human polyclonal antitetanus globulin in both in vitro and in vivo tests.
Conclusions:
- Hybridoma technology can be effectively applied to generate cells producing anti-tetanus toxoid antibodies.
- The characterized hybrid cells show potential as a source for therapeutic or diagnostic agents against tetanus.