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Fractionation of human serum proteins by immobilized metal affinity chromatography
Abstract:
Human serum was fractionated on zinc and cadmium iminodiacetate and tris(carboxymethyl)ethylenediamine chelates. Zinc iminodiacetate was found to be useful in the isolation of hemopexin and alpha 2-macroglobulin. The chromatographic behaviour of serum proteins on zinc iminodiacetate could be modulated by the nature of the eluent; a sulphate-containing solvent increased the binding of serum proteins to immobilized metal ions.
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