Binding parameters of interactions of monomer-polymer systems based on quenching of their completely overlapped...

A theory is presented which describes the interactions between completely overlapping fluorescing monomer-polymer systems and affords a method to calculate binding parameters. The theory is based on the assumptions that the complex formed from the fluorescing binding site and the monomer molecule does not fluoresce and that the fluorescence intensity is linearly related to concentration. The relationships derived from this theory have provided a sensitive and easy method for calculating the number of binding sites on a polymer molecule from only three emission-intensity values. The theory has been tested on systems containing trimethoprim-serum albumins and found to yield results which are consistent with a curve-fitting approach to the experimental data. This reflects the validity of the theoretical model presented and the various assumptions and approximations made.