Related Experiment Video
Updated: Aug 19, 2026

A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening
Published on: October 16, 2015
Determination of flurbiprofen in human serum by reverse-phase high-performance liquid chromatography with
Abstract:
A reverse-phase high-performance liquid chromatographic method is described for the determination of flurbiprofen in human serum. Flurbiprofen is extracted from hydrochloric acid-acidified serum with pentane-ether (80:20). An octadecylsilane column was used with a mobile phase of acetonitrile-water-phosphoric acid (650:350:0.5, v/v/v). A fluorescence detector with excitation at 250 nm and emission at 315 nm provided a quantifiable peak for 0.1 microgram/mL of flurbiprofen in 0.5 mL of plasma. A comparison between UV and fluorescence detection systems is presented. The method is applicable to human bioavailability and pharmacokinetic studies with flurbiprofen.
More Related Videos
09:04Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow
Published on: April 18, 2019
08:33Radiosynthesis of 1-(2-[18F]Fluoroethyl)-L-Tryptophan using a One-pot, Two-step Protocol
Published on: September 21, 2021
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Types of Detectors