High-performance liquid chromatographic method for the determination of tiflamizole in plasma

A sensitive, specific, high-performance liquid chromatographic procedure was developed for the measurement of plasma tiflamizole levels. Acidic plasma samples were extracted with three volumes of ether. The ether extracts were combined and evaporated to dryness. The residue was dissolved in acetonitrile, washed with hexane, and the acetonitrile was evaporated to dryness. The residue was dissolved in 0.5 mL of mobile phase consisting of acetonitrile and 0.007 M pH 3 sodium phosphate buffer (70:30, v/v) and then chromatographed on a octadecylsilane bonded microparticulate silica column. The assay is specific, precise, accurate, and can measure 10 ng of tiflamizole in 5 mL of plasma. The method was applied to human pharmacokinetic studies.