Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An empirical method for converting nucleolar counts to neuronal numbers.

R E Coggeshall, K Chung, D Greenwood

    Journal of Neuroscience Methods
    |December 1, 1984
    PubMed
    Summary

    This study introduces a simple formula to accurately estimate neuron numbers from nucleolar profile counts. The method corrects for multiple nucleoli per nucleus, improving neuronal counting accuracy.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Development of the Manchester framework for the evaluation of emergency department pharmacy services.

    International journal of clinical pharmacy·2022
    Same author

    The description and definition of Emergency Department Pharmacist Practitioners in the United Kingdom (the ENDPAPER study).

    International journal of clinical pharmacy·2019
    Same author

    Long-term health effects of chemical warfare agents on children following a single heavy exposure.

    Human & experimental toxicology·2017
    Same author

    Foods and beverages and colorectal cancer risk: a systematic review and meta-analysis of cohort studies, an update of the evidence of the WCRF-AICR Continuous Update Project.

    Annals of oncology : official journal of the European Society for Medical Oncology·2017
    Same author

    Fruits, vegetables and lung cancer risk: a systematic review and meta-analysis.

    Annals of oncology : official journal of the European Society for Medical Oncology·2015
    Same author

    Inflammatory cytokine receptor blockade in a rodent model of mild traumatic brain injury.

    Journal of neuroscience research·2015

    Area of Science:

    • Neuroscience
    • Quantitative Biology
    • Cell Biology

    Background:

    • Estimating neuronal numbers is crucial for understanding brain structure and function.
    • Counting nucleolar profiles is a common method, but accuracy is challenged by multiple nucleoli per nucleus.
    • Existing methods lack a simple, generalizable approach to correct for these counting discrepancies.

    Purpose of the Study:

    • To develop a straightforward and broadly applicable formula for estimating true neuronal numbers from nucleolar profile counts.
    • To address the challenge of multiple nucleoli within a single nucleus affecting accurate cell enumeration.

    Main Methods:

    • Proposed a correction factor formula: N = n X [N(c.f.)/n(c.f.)].
    • N represents the true neuron count, n is the observed nucleolar profile count.

    Related Experiment Videos

  • N(c.f.) and n(c.f.) are counts from a representative neuron sample used to estimate the correction factor.

    • Main Results:

    • The proposed formula provides a simple and generalizable method for neuronal number estimation.
    • The empirical correction factor accounts for variations like multiple or split nucleoli, and sectioning artifacts.
    • This approach calibrates for factors causing deviations between observed nucleolar profiles and true neuron counts.

    Conclusions:

    • The developed formula offers a practical solution for accurate neuronal population sizing.
    • Its empirical nature allows adaptation to various biological and technical factors influencing nucleolar profile counts.
    • This method enhances the reliability of neuronal counting in neuroscience research.