Effects of organic solvents on lipase for fat splitting

The effect of organic solvents on the stability and catalytic activity of the microbial lipase from Candida rugosa for hydrolysis of triglyceride (fat splitting) has been examined. The solvents examined were 5 hydrocarbons (n-hexane, n-heptane, n-octane, iso-octane and cyclohexane) and 3 ethers (diethyl-ether, diisopropylether and di-n-butylether). The results revealed that iso-octane and cyclohexane are superior to the other solvents examined for enzymatic fat splitting in organic solvent systems.