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Related Experiment Videos

Description of analytical problems arising from elevated serum solids.

M W McGowan, J D Artiss, B Zak

    Analytical Biochemistry
    |November 1, 1984
    PubMed
    Summary
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    High solids in biological fluids like serum cause inaccurate spectrophotometric measurements due to turbidity and dilution effects. This review addresses challenges in analyzing hyperlipemic and hyperproteinemic samples.

    Area of Science:

    • Clinical Chemistry
    • Analytical Biochemistry
    • Spectrophotometry

    Background:

    • Elevated solids (lipids, proteins) in biological fluids cause turbidity and dilution.
    • Turbidity leads to optical aberrations in spectrophotometric measurements.
    • Solids act as diluents, reducing true plasma volume and causing short sampling errors.

    Purpose of the Study:

    • To review challenges in measuring analytes in hyperlipemic/hyperproteinemic specimens.
    • To discuss issues related to short sampling caused by high solids content.
    • To explore methods for correcting analytical errors in such samples.

    Main Methods:

    • Review of existing literature on analytical interferences in biological fluids.
    • Discussion of mathematical corrections, lipid extraction, and ultracentrifugation techniques.

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  • Analysis of reference base value differences and use of ratios.

    • Main Results:

    • High solids content significantly impacts assay values, leading to underestimation.
    • Short sampling errors occur due to both dilution and increased viscosity.
    • Neglecting corrective actions for solids volume leads to inaccurate spectrophotometric determinations.

    Conclusions:

    • Accurate analyte measurement in hyperlipemic/hyperproteinemic samples requires addressing solids-related interferences.
    • Corrective mathematics, lipid removal, or ultracentrifugation are potential solutions.
    • Standardized methods considering solids volume are crucial for reliable clinical chemistry results.