Detection of SCE in rodent cells using the activated charcoal bromodeoxyuridine system

An in vivo method of sister chromatid differentiation based on the intraperitoneal (ip) injection of bromodeoxyuridine (BrdUrd) previously adsorbed to activated charcoal is described, as well as the protocols for the use of this method in spermatogonial, salivary gland and bone marrow cells, will be presented. Results on sister chromatid exchange (SCE) induction obtained in bone marrow cells using well-known mutagens, especially cyclophosphamide, are reported, and a comparison is made with the results obtained by other authors using different methods of BrdUrd administration. The usefulness of this in vivo system for chemical mutagen detection and for chemical mutagen action on SCE induction is discussed.