Lysosomal acid deoxyribonuclease from vervet monkey livers--II. Enzymic properties

Primate liver lysosomal acid DNase is an endonucleolytic enzyme. The enzyme has both 3'- and 5'-nucleotidohydrolase activities. The oligonucleotides produced by DNase are polymers mainly about 30 mononucleotides long. The Arrhenius plot shows a discontinuity with a transition temperature at 47 degrees C, with an activation energy of 107 kJ/mol below and 67 kJ/mol above this temperature. The activation enthalpy is 104 kJ/mol and the entropy -0.498 kJ/mol/K. The enzyme is subject to substrate inhibition and the Km value is 159 X 10(-3) mM DNA-P.