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Effect of nuclear magnetic resonance on chromosomes of mouse bone marrow cells
Abstract:
Three groups of six male Balb/c mice, subjected to 30 MHz continuous wave NMR exposure in a static magnetic field of 7.05 K Gauss for one hour, were each compared to another group of ten unexposed mice with respect to chromatid and chromosomal aberrations. The exposed groups were sacrificed at two hours, 24 hours and 48 hours following NMR exposure respectively. Control mice were sacrificed 24 hours after sham-exposure. All groups had approximately 0.02 apparent aberrations per cell. These apparent aberrations were in the form of metacentric chromosomes, possibly resulting from a union of chromosomes at their centromeres or possibly simply chromosomes in association. The results are consistent with earlier in vitro findings that NMR exposure causes no adverse cytogenetic effects.
Insights
Nuclear Magnetic Resonance (NMR) exposure did not cause adverse cytogenetic effects in mice. Analysis of chromatid and chromosomal aberrations showed no significant differences between exposed and control groups.
Area of Science:
- Biophysics
- Genetics
- Toxicology
Background:
- Nuclear Magnetic Resonance (NMR) is increasingly used in research and medicine.
- Understanding potential biological effects, particularly cytogenetic impacts, is crucial.
Purpose of the Study:
- To investigate the potential cytogenetic effects of 30 MHz continuous wave NMR exposure in vivo.
- To assess chromatid and chromosomal aberrations in male Balb/c mice following NMR exposure.
Main Methods:
- Male Balb/c mice were exposed to 30 MHz continuous wave NMR in a 7.05 K Gauss static magnetic field for one hour.
- Exposed groups were sacrificed at 2, 24, and 48 hours post-exposure.
- Chromatid and chromosomal aberrations were analyzed and compared to sham-exposed control groups.
Main Results:
- All groups, including exposed and control, exhibited a low rate of apparent aberrations (approximately 0.02 per cell).
- Apparent aberrations were characterized as metacentric chromosomes or chromosome associations.
- No significant increase in aberrations was observed in NMR-exposed mice compared to controls at any time point.
Conclusions:
- In vivo NMR exposure at 30 MHz and 7.05 K Gauss does not induce adverse cytogenetic effects in male Balb/c mice.
- The findings support previous in vitro studies indicating NMR's safety regarding genotoxicity.