Affinity sorbents containing benzamidine derivatives for biomedical application

Affinity sorbents for trypsin have been obtained by immobilization of 4-amidinophenylalkylcarboxylic acids on macroporous silica gel. Frontal chromatography was used to measure the dissociation constants of the trypsin-immobilized inhibitor complexes (Kd). The Kd value has been found to increase 50-100 fold on immobilization. A commercial trypsin preparation was purified on the obtained biospecific sorbent. Applicability of the affinity sorbent containing 4-amidinophenylacetic acid was demonstrated by hemoperfusion in dogs with acute pancreatitis. The sorbent with immobilized low molecular mass trypsin inhibitor has the capacity to greatly reduce the level of proteases in blood without altering the concentration of protease inhibitors: this results in efficient treatment.