Effect of oxygen pressure during culture on survival of mouse thyroid allografts

A marked increase in the percentage of mouse thyroids that retained function 20 days after transplantation across a major histocompatibility barrier and the percentage that lacked generalized infiltration was observed when the grafts received hyperbaric oxygen during a 4-day culture period. Perfusion of the donor animal before thyroidotomy and the addition of fetal calf serum to the culture medium did not have a significant effect on graft survival, but the percentage of grafts lacking generalized infiltration was slightly increased by the addition of hydrocortisone to the culture medium.