Mechanical behavior of pressurized in vitro prearteriolar vessels determined with a video system

The muscular resistance arteries of the mesentery and brain serve two different control functions in the cardiovascular system. The former are representative vessels of vascular beds that influence total peripheral resistance and blood pressure; the latter are a good model of vessels in beds that demonstrate blood flow autoregulation. Our purpose was to develop a versatile myographic system appropriate for the in vitro study of 75-250 micron diameter vessels and to explore different physiological properties of cerebral and mesenteric arteries. In this paper the system is described in detail, examples of its use in determining the dynamic responses of the vessels to electrical stimulation are provided, and certain measures indicative of the extent of myogenic behavior are characterized. Cylindrical artery segments about 3-mm long were dissected from Wistar-Kyoto rats and mounted in a chamber filled with physiological saline solution maintained at 37 degrees C. The same solution was perfused via a syringe into one end of the vessel through a microcannula. The other end was then occluded so that experiments could be made over a wide range of transmural pressures without flow. The vessel was viewed through a microscope coupled with a TV camera, and the video output signal of a selected scan line was processed by an electronic dimension analyzing system. This permitted simultaneous digital presentation and analog voltage outputs of the vessel wall thicknesses and lumen diameter. We further incorporated servo control of the syringe using a motor drive. In this way, vessel tests could be carried out at constant pressure or constant diameter, and vessel responses could be obtained following either pressure or diameter command signals. Using the methods presented in this study, small vessels can be maintained under conditions that approximate their in vivo state more closely than other in vitro techniques using ring segments on wires. We also find that the opto-electronic instrumentation is ideally suited for studying the dynamic vessel properties that underlie the control of vascular smooth muscle.