Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Junction formation in aggregated embryonal carcinoma cells.

J M Strum, S Kartha, J S Felix

    Developmental Biology
    |September 1, 1984
    PubMed
    Summary

    Supplemental calcium induces H6 mouse embryonal carcinoma cell compaction, similar to embryonic compaction. Gap junction size significantly increases during compaction, while tight junctions and desmosomes form later, indicating calcium-driven cell flattening independent of junction formation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A novel functional screening method for generation of a synthetic microbial community: Case study with control of Fusarium wilt in pigeonpea.

    Plant biology (Stuttgart, Germany)·2025
    Same author

    Response to the Letter to the Editor: "Intra-articular Injection of Mono-iodoacetate and TMJ Osteoarthritis".

    Journal of dental research·2019
    Same author

    Experimental Methods to Inform Diagnostic Approaches for Painful TMJ Osteoarthritis.

    Journal of dental research·2019
    Same author

    Intra-articular nerve growth factor regulates development, but not maintenance, of injury-induced facet joint pain & spinal neuronal hypersensitivity.

    Osteoarthritis and cartilage·2015
    Same author

    Identification and migration of degradation compounds from irradiation of multilayer polyamide 6 films for meat foodstuffs and cheese.

    Analytical and bioanalytical chemistry·2008
    Same author

    Regulation of cyclin D(1) expression and DNA synthesis by phosphatidylinositol 3-kinase in airway smooth muscle cells.

    American journal of respiratory cell and molecular biology·2000

    Area of Science:

    • Developmental Biology
    • Cell Biology
    • Biochemistry

    Background:

    • Embryonic compaction is crucial for mammalian development.
    • Cell-cell junctions, particularly gap junctions, are implicated in compaction.
    • H6 mouse embryonal carcinoma cells mimic early embryonic development.

    Purpose of the Study:

    • To investigate the role and pattern of cell junction formation during calcium-induced H6 cell aggregation and compaction.
    • To determine the relationship between gap junction formation and the morphological changes associated with compaction.
    • To elucidate the role of calcium in H6 cell compaction and junction development.

    Main Methods:

    • H6 mouse embryonal carcinoma cell culture and aggregation.
    • Induction of cell compaction using supplemental calcium.

    Related Experiment Videos

  • Quantitative analysis of freeze fracture replicas of cell junctions (gap junctions, tight junctions, desmosomes).
  • Time-course analysis of junction formation at 12, 24, and 48 hours of aggregation.

    • Main Results:

    • Gap junctions were abundant in both uncompacted and compacted H6 cell aggregates.
    • A 20-fold increase in the largest gap junction size was observed in compacted aggregates by 24 hours, detectable as early as 12 hours.
    • Tight junctions and desmosomes showed initial formation in compacted aggregates at 12 hours and were more evident after 48 hours.
    • Compaction morphology was observed independently of gap junction formation frequency.
    • Calcium-induced cell flattening appears to be the primary driver of compaction.

    Conclusions:

    • Gap junction formation is not dependent on cell flattening or the compacted morphology in H6 cells.
    • Compaction in H6 cells is likely driven by calcium-induced cell flattening, possibly involving cytoskeletal elements.
    • The calcium-dependent nature of H6 cell aggregation and compaction allows for independent analysis of developmental processes.